PERINEURAL CAPSAICIN INDUCES THE UPTAKE AND TRANSGANGLIONIC TRANSPORT OF CHOLERATOXIN B SUBUNIT BY NOCICEPTIVE C-FIBER PRIMARY AFFERENT NEURONS
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Tartalom: http://real.mtak.hu/28913/
Archívum: MTA Könyvtár
Gyűjtemény: Status = Submitted

Type = Article
Cím:
PERINEURAL CAPSAICIN INDUCES THE UPTAKE AND TRANSGANGLIONIC TRANSPORT OF CHOLERATOXIN B SUBUNIT BY NOCICEPTIVE C-FIBER PRIMARY AFFERENT NEURONS
Létrehozó:
Oszlács, Orsolya
Jancsó, Gábor
Kis, Gyöngyi
Dux, Mária
Sántha, Péter
Dátum:
2015
Téma:
R1 Medicine (General) / orvostudomány általában
Tartalmi leírás:
The distribution of spinal primary afferent terminals labeled
transganglionically with the choleratoxin B subunit (CTB) or its
conjugates changes profoundly after perineural treatment with capsaicin.
Injection of CTB conjugated with horseradish peroxidase (HRP) into an
intact nerve labels somatotopically related areas in the ipsilateral
dorsal horn with the exceptions of the marginal zone and the substantia
gelatinosa, whereas injection of this tracer into a capsaicin-pretreated
nerve also results in massive labeling of these most superficial layers
of the dorsal horn. The present study was initiated to clarify the role
of C-fiber primary afferent neurons in this phenomenon. In L5 dorsal root
ganglia, analysis of the size frequency distribution of neurons labeled
after injection of CTB-HRP into the ipsilateral sciatic nerve treated
previously with capsaicin or resiniferatoxin revealed a significant
increase in the proportion of small neurons. In the spinal dorsal horn,
capsaicin or resiniferatoxin pretreatment resulted in intense CTB-HRP
labeling of the marginal zone and the substantia gelatinosa. Electron
microscopic histochemistry disclosed a dramatic, ~ 10-fold increase in
the proportion of CTB-HRP-labeled unmyelinated dorsal root axons
following perineural capsaicin or resiniferatoxin. The present results
indicate that CTB-HRP labeling of C-fiber dorsal root ganglion neurons
and their central terminals after perineural treatment with vanilloid
compounds may be explained by their phenotypic switch rather than a
sprouting response of thick myelinated spinal afferents which, in an
intact nerve, can be labeled selectively with CTB-HRP. The findings also
suggest a role for GM1 ganglioside in the modulation of nociceptor
function and pain.
Típus:
Article
NonPeerReviewed
Formátum:
text
Azonosító:
Oszlács, Orsolya and Jancsó, Gábor and Kis, Gyöngyi and Dux, Mária and Sántha, Péter (2015) PERINEURAL CAPSAICIN INDUCES THE UPTAKE AND TRANSGANGLIONIC TRANSPORT OF CHOLERATOXIN B SUBUNIT BY NOCICEPTIVE C-FIBER PRIMARY AFFERENT NEURONS. NEUROSCIENCE. ISSN 0306-4522 (Submitted)
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