UVB-dependent changes in the expression of fast-responding early genes is modulated by huCOP1 in keratinocytes
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Tartalom: http://real.mtak.hu/28024/
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published

Type = Article
Cím:
UVB-dependent changes in the expression of fast-responding early genes is modulated by huCOP1 in keratinocytes
Létrehozó:
Fazekas, Barbara
PolyĂĄnka, Hilda
Bebes, Attila
Tax, G.
SzabĂł, KornĂŠlia
Farkas, Katalin
Kinyó, Ágnes
Nagy, Ferenc
KemĂŠny, Lajos
SzĂŠll, MĂĄrta
Ádám, Éva
Kiadó:
Elsevier Science Publishers B.V.
Dátum:
2014-11
Téma:
RL Dermatology / bőrgyógyászat
Tartalmi leírás:
Ultraviolet (UV) B is the most prominent physical carcinogen in the environment leading to the development of various skin cancers. We have previously demonstrated that the human ortholog of the Arabidopsis thaliana constitutive photomorphogenesis 1 (COP1) protein, huCOP1, is expressed in keratinocytes in a UVB-regulated manner and is a negative regulator of p53 as a posttranslational modifier. However, it was not known whether huCOP1 plays a role in mediating the UVB-induced early transcriptional responses of human keratinocytes. In this study, we report that stable siRNA-mediated silencing of huCOP1 affects the UVB response of several genes within 2 h of irradiation, indicating that altered huCOP1 expression sensitizes the cells toward UVB. Pathway analysis identified a molecular network in which 13 of the 30 examined UVB-regulated genes were organized around three central proteins. Since the expression of the investigated genes was upregulated by UVB in the siCOP1 cell line, we hypothesize that huCOP1 is a repressor of the identified pathway. Several members of the network have been implicated previously in the pathogenesis of non-melanoma skin cancers; therefore, clarifying the role of huCOP1 in these skin diseases may have clinical relevance in the future.
Nyelv:
magyar
Típus:
Article
NonPeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Fazekas, Barbara and Polyánka, Hilda and Bebes, Attila and Tax, G. and Szabó, Kornélia and Farkas, Katalin and Kinyó, Ágnes and Nagy, Ferenc and Kemény, Lajos and Széll, Márta and Ádám, Éva (2014) UVB-dependent changes in the expression of fast-responding early genes is modulated by huCOP1 in keratinocytes. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 140. pp. 215-222. ISSN 1011-1344
Kapcsolat:
doi: 10.1016/j.jphotobiol.2014.08.002