| Tartalom: |
http://www.nature.com/ncomms/2015/150420/ncomms7557/full/... |
| Archívum: |
MTA Könyvtár |
| Gyűjtemény: |
Status = Published
Type = Article
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| Cím: |
Tonic endocannabinoid-mediated modulation of
GABA release is independent of the CB1 content
of axon terminals
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| Létrehozó: |
Lenkey, Nora
Kirizs, Tekla
Holderith, Noemi
Máté, Zoltán
Szabó, Gábor
Vizy, E. Sylvester
Hájos, Norbert
Nusser, Zoltan
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| Dátum: |
2015-04-20
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| Téma: |
Q1 Science (General) / természettudomány általában
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| Tartalmi leírás: |
The release of GABA from cholecystokinin-containing interneurons is modulated by type-1 cannabinoid receptors (CB1). Here we tested the hypothesis that the strength of CB1-mediated modulation of GABA release is related to the CB1 content of axon terminals. Basket cell boutons have on average 78% higher CB1 content than those of dendritic-layer-innervating (DLI) cells, a consequence of larger bouton surface and higher CB1 density. The CB1 antagonist AM251 caused a 54% increase in action potential-evoked [Ca2+] in boutons of basket cells, but not in DLI cells. However, the effect of AM251 did not correlate with CB1 immunoreactivity of individual boutons. Moreover, a CB1 agonist decreased [Ca2+] in a cell type- and CB1-content-independent manner. Replica immunogold labelling demonstrated the colocalization of CB1 with the Cav2.2 Ca2+ channel subunit. Our data suggest that only a subpopulation of CB1s, within nanometre distances from their target Cav2.2 channels, are responsible for endocannabinoid-mediated modulation of GABA release.
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| Típus: |
Article
PeerReviewed
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| Formátum: |
text
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| Azonosító: |
Lenkey, Nora and Kirizs, Tekla and Holderith, Noemi and Máté, Zoltán and Szabó, Gábor and Vizy, E. Sylvester and Hájos, Norbert and Nusser, Zoltan (2015) Tonic endocannabinoid-mediated modulation of GABA release is independent of the CB1 content of axon terminals. Nature communications, 6 (6557). pp. 1-14. ISSN 2041-1723
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