Novel role for galectin-1 in T-cells under physiological and pathological conditions
  • Metaadatok
Tartalom: http://real.mtak.hu/27736/
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published


Type = Article
Cím:
Novel role for galectin-1 in T-cells under physiological and pathological conditions
Létrehozó:
Deák, Magdolna
Hornung, Ákos
Novák, Julianna
Demydenko, Dmytro
Szabó, Enikő
Czibula, Ágnes
Fajka-Boja, Roberta
Kriston-Pál, Éva
Monostori, Éva
Kovács, László
Dátum:
2015
Téma:
QH301 Biology / biológia
QR180 Immunology / immunológia
Tartalmi leírás:
Secreted, extracellular galectin-1 (exGal-1) but not intracellular Gal-1 (inGal-1) has been described as a strong immunosuppressive protein due to its major activity of inducing apoptosis of activated T-cells. It has previously been reported that T-cells express Gal-1 upon activation, however its participation in T-cell functions has remained largely elusive. To determine function of Gal-1 expressed by activated T-cells we have carried out a series of experiments. We have shown that Gal-1, expressed in Gal-1-transgenic Jurkat cells or in activated T-cells, remained intracellularly indicating that Gal-1-induced T-cell death was not a result of an autocrine effect of the de novo expressed Gal-1. Rather, a particular consequence of the inGal-1 expression was that T-cells became more sensitive to exGal-1 added either as a soluble protein or bound to the surface of a Gal-1-secreting effector cell. This was also verified when the susceptibility of activated T-cells from wild type or Gal-1 knockout mice to Gal-1-induced apoptosis were compared. Murine T-cells expressing Gal-1 were more sensitive to the cytotoxicity of the exGal-1 than their Gal-1 knockout counterparts. We also conducted a study with activated T-cells from patients with systemic lupus erythematosus (SLE), a disease in which dysregulated T-cell apoptosis has been well described. SLE T-cells expressed lower amounts of Gal-1 than healthy T-cells and were less sensitive to exGal-1. These results suggested a novel role of inGal-1 in T-cells as a regulator of T-cell response to exGal-1, and its likely contribution to the mechanism in T-cell apoptosis deficiency in lupus.
Típus:
Article
PeerReviewed
Formátum:
text
Azonosító:
Deák, Magdolna and Hornung, Ákos and Novák, Julianna and Demydenko, Dmytro and Szabó, Enikő and Czibula, Ágnes and Fajka-Boja, Roberta and Kriston-Pál, Éva and Monostori, Éva and Kovács, László (2015) Novel role for galectin-1 in T-cells under physiological and pathological conditions. IMMUNOBIOLOGY, 220 (4). pp. 483-489. ISSN 0171-2985
Kapcsolat: