A Cucumber Mosaic Virus Based Expression System for the Production of Porcine Circovirus Specific Vaccines
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Tartalom: http://real.mtak.hu/26589/
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published



Type = Article
Cím:
A Cucumber Mosaic Virus Based Expression System for the Production of Porcine Circovirus Specific Vaccines
Létrehozó:
Gellért, Ákos
Salánki, Katalin
Tombácz, Kata
Tuboly, Tamás
Balázs, Ervin
Dátum:
2012
Téma:
QP Physiology / élettan
QR355 Virology / víruskutatás
SB Plant culture / növénytermesztés
SV Veterinary science / állatorvostudomány
Tartalmi leírás:
Potential porcine circovirus type 2 (PCV2) capsid protein epitopes, suitable for expression on the surface of cucumber
mosaic virus (CMV) particles were determined by a thorough analysis of the predicted PCV capsid protein structure. The ab
initio protein structure prediction was carried out with fold recognition and threading methods. The putative PCV epitopes
were selected on the basis of PCV virion models and integrated into the plant virus coat protein, after amino acid position
131. The recombinants were tested for infectivity and stability on different Nicotiana species and stable recombinant virus
particles were purified. The particles were tested for their ability to bind to PCV induced porcine antibodies and used for
specific antibody induction in mice and pigs. The results showed that PCV epitopes expressed on the CMV surface were
recognized by the porcine antibodies and they were also able to induce PCV specific antibody response. Challenge
experiment with PCV2 carried out in immunized pigs showed partial protection against the infection. Based on these results
it was concluded that specific antiviral vaccine production for the given pathogen was feasible, offering an inexpensive way
for the mass production of such vaccines.
Típus:
Article
PeerReviewed
Formátum:
text
Azonosító:
Gellért, Ákos and Salánki, Katalin and Tombácz, Kata and Tuboly, Tamás and Balázs, Ervin (2012) A Cucumber Mosaic Virus Based Expression System for the Production of Porcine Circovirus Specific Vaccines. PLOS ONE, 7 (12). e52688. ISSN 1932-6203
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