| Tartalom: |
http://dx.doi.org/10.1007/s12033-015-9874-1 |
| Archívum: |
MTA Könyvtár |
| Gyűjtemény: |
Status = Published
Type = Article
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| Cím: |
Xylan-Degrading Catalytic Flagellar Nanorods
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| Létrehozó: |
Klein, A.
Szabo, V.
Kovacs, M.
Patkó, Dániel
Toth, B.
Vonderviszt, Ferenc
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| Dátum: |
2015
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| Téma: |
QH3015 Molecular biology / molekuláris biológia
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| Tartalmi leírás: |
Flagellin, the main component of flagellar filaments, is a protein possessing polymerization ability. In this work, a novel fusion construct of xylanase A from B. subtilis and Salmonella flagellin was created which is applicable to build xylan-degrading catalytic nanorods of high stability. The FliC-XynA chimera when overexpressed in a flagellin deficient Salmonella host strain was secreted into the culture medium by the flagellum-specific export machinery allowing easy purification. Filamentous assemblies displaying high surface density of catalytic sites were produced by ammonium sulfate-induced polymerization. FliC-XynA nanorods were resistant to proteolytic degradation and preserved their enzymatic activity for a long period of time. Furnishing enzymes with self-assembling ability to build catalytic nanorods offers a promising alternative approach to enzyme immobilization onto nanostructured synthetic scaffolds.
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| Típus: |
Article
PeerReviewed
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| Formátum: |
text
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| Azonosító: |
Klein, A. and Szabo, V. and Kovacs, M. and Patkó, Dániel and Toth, B. and Vonderviszt, Ferenc (2015) Xylan-Degrading Catalytic Flagellar Nanorods. MOLECULAR BIOTECHNOLOGY, 57 (9). pp. 814-819. ISSN 1073-6085
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