Possible antagonistic effect of three fusarium mycotoxins on genotoxicity of spermatozoa of breeding rabbit bucks
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Type = Conference or Workshop Item
Cím:
Possible antagonistic effect of three fusarium mycotoxins on genotoxicity of spermatozoa of breeding rabbit bucks
Létrehozó:
Kachlek, Mariam Lilia
Szabó-Fodor, Judit
Blochné Bodnár, Zsófia
Kovács, Melinda
Dátum:
2015-04-13
Téma:
QP Physiology / élettan
Tartalmi leírás:
Fusarium species can produce several mycotoxins e.g. FB1, DON and ZEA
which are frequent contaminants of cereals intended either for human or
animal consumption. F. verticillioides and F. proliferatum produce FB1
whereas F. graminearum is the main producer of DON and ZEA resulting in
co-occurrence.
Although multimycotoxin contamination occurs very often, most studies
focus on single effects. Moreover the mycotoxins? concentrations used in
the experimental diets are usually high and unlikely to occur in nature. Data
on the effect of combined mycotoxins consumption in low dosages
(according to EU recommendations) are scarce. In addition there are no
studies assessing the genotoxicity of these Fusarium toxins on reproduction
system and especially on spermatozoa after in vivo exposure.
The aim of this study was to investigate the genotoxicity of FB1, DON and
ZEA alone as well as in combination in low dosages on spermatozoa derived
from breeding rabbit bucks after in vivo treatment.
The rabbit bucks were fed for 65 days with the experimental diets; Control
(C), FB1 (F), ZEA+DON (ZD) and FB1+ZEA+DON (FZD), the concentrations
were 0 mg/kg, 5 mg/kg, 0.25+1 mg/kg and 5+0.25+1 mg/kg respectively. On
day 65, semen was collected and Comet assay was performed to assess the
genotoxicity (DNA damage).
According to the Comet assay results, F treatment resulted in significantly
less 0 comets compared to other treatments. Regarding score 1 all toxin
treatments had similar proportions. As for score 2, F had significantly
increased number of cells compared to FZD. Few cells had score of 3
(maximum 0.625%) whereas no cells with score 4 were found. It can be
concluded that the combined toxins act rather antagonistically than FB1
alone since the combination led to lower DNA damage (higher score 0 and
lower score 2).
This is the first time interactive genotoxic effects of FB1, DON and ZEA on
rabbit spermatozoa are reported.
Típus:
Conference or Workshop Item
NonPeerReviewed
Formátum:
text
Azonosító:
Kachlek, Mariam Lilia and Szabó-Fodor, Judit and Blochné Bodnár, Zsófia and Kovács, Melinda (2015) Possible antagonistic effect of three fusarium mycotoxins on genotoxicity of spermatozoa of breeding rabbit bucks. In: International Conference on Food Contaminants: challenges in chemical mixtures, 2015.04.13-2015.04.14., Lisszabon, Portugália.
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