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Kapcsolat
Visualization of Calcium Dynamics in Kidney Proximal Tubules |
Tartalom: | http://real.mtak.hu/26249/ |
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Archívum: | MTA Könyvtár |
Gyűjtemény: |
Status = Published
Type = Article |
Cím: |
Visualization of Calcium Dynamics in Kidney Proximal Tubules
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Létrehozó: |
SzebĂŠnyi, KornĂŠlia
FĂźredi, AndrĂĄs
Kolacsek, Orsolya
CsohĂĄny, RĂłzsa
PrĂłkai, Ăgnes
Kis-Petik, Katalin
SzabĂł, Attila
BĹsze, Zsuzsanna
Bender, BalĂĄzs
TĂłvĂĄri, JĂłzsef
Enyedi, Ăgnes
OrbĂĄn, TamĂĄs I.
ApĂĄti, Ăgota
Sarkadi, BalĂĄzs
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Dátum: |
2015
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Téma: |
RJ Pediatrics / gyermekgyĂłgyĂĄszat
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Tartalmi leírás: |
Intrarenal changes in cytoplasmic calcium levels have a key role in determining pathologic and pharmacologic responses in major kidney diseases. However, cell-specific delivery of calcium-sensitive probes in vivo remains problematic. We generated a transgenic rat stably expressing the green fluorescent protein-calmodulin-based genetically encoded calcium indicator (GCaMP2) predominantly in the kidney proximal tubules. The transposon-based method used allowed the generation of homozygous transgenic rats containing one copy of the transgene per allele with a defined insertion pattern, without genetic or phenotypic alterations. We applied in vitro confocal and in vivo two-photon microscopy to examine basal calcium levels and ligand- and drug-induced alterations in these levels in proximal tubular epithelial cells. Notably, renal ischemia induced a transient increase in cellular calcium, and reperfusion resulted in a secondary calcium load, which was significantly decreased by systemic administration of specific blockers of the angiotensin receptor and the Na-Ca exchanger. The parallel examination of in vivo cellular calcium dynamics and renal circulation by fluorescent probes opens new possibilities for physiologic and pharmacologic investigations.
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Nyelv: |
angol
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Típus: |
Article
PeerReviewed
info:eu-repo/semantics/article
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Formátum: |
text
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Azonosító: |
SzebĂŠnyi, KornĂŠlia and FĂźredi, AndrĂĄs and Kolacsek, Orsolya and CsohĂĄny, RĂłzsa and PrĂłkai, Ăgnes and Kis-Petik, Katalin and SzabĂł, Attila and BĹsze, Zsuzsanna and Bender, BalĂĄzs and TĂłvĂĄri, JĂłzsef and Enyedi, Ăgnes and OrbĂĄn, TamĂĄs I. and ApĂĄti, Ăgota and Sarkadi, BalĂĄzs (2015) Visualization of Calcium Dynamics in Kidney Proximal Tubules. JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 26 (xx). xx-xx. ISSN 1046-6673
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Kapcsolat: |
MTMT:2870811; doi:10.1681/ASN.2014070705
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