A "yellow" laccase with "blue" spectroscopic features, from Sclerotinia sclerotiorum
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Tartalom: http://ac.els-cdn.com/S1359511312001092/1-s2.0-S135951131...
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published

Type = Article
Cím:
A "yellow" laccase with "blue" spectroscopic features, from Sclerotinia sclerotiorum
Létrehozó:
Mot, A. C.
Parvu, M.
Damian, G.
Irimie, F. D.
Darula, Zsuzsanna
Medzihradszky F., Katalin
Brem, Balázs
Dátum:
2012
Téma:
QH3011 Biochemistry / biokémia
Tartalmi leírás:
Reported here are the production, purification and characterization of a laccase from the phytophathogenic fungus Sclerotinia sclerotiorum. This laccase is identified by mass spectrometry with a sequence coverage of 74.9% (458/577 AA) revealing that the protein is identical or highly homologous to a predicted oxidoreductase from this species (A7EM18 in the Uniprot database); the closest homologous protein previously isolated from a fungus is the Melanocarpus albomyces, with only 35% identity. The UV-vis spectral features of this laccase classify it as a "yellow" one. The EPR spectrum nevertheless demonstrates resemblance to blue laccases - including the type 1 center not detectable in UV-vis spectra. The presence of type 3 coppers was proven by fluorescence spectrum and by 330 nm band in UV-vis. The purified laccase has an apparent molecular mass of 70 kDa and appears as a monomer. The values of K-M and k(cat) were determined for ABTS, 2,6-dimethoxyphenol, p-phenylenediamine and guaicol and are typical of a laccase. The optimal pH value is around 4 except for ABTS, for which activity is linearly increasing with acidity. The high laccase activity in liquid culture makes S. sclerotiorum a useful source of laccase for practical applications. (C) 2012 Elsevier Ltd. All rights reserved.
Típus:
Article
PeerReviewed
Formátum:
text
Azonosító:
Mot, A. C. and Parvu, M. and Damian, G. and Irimie, F. D. and Darula, Zsuzsanna and Medzihradszky F., Katalin and Brem, Balázs (2012) A "yellow" laccase with "blue" spectroscopic features, from Sclerotinia sclerotiorum. PROCESS BIOCHEMISTRY, 47 (6). pp. 968-975. ISSN 1359-5113
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