Immunohistochemical detection of raf protein kinase in cerebral cortical areas of adult guinea pigs and rats
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Type = Article
Cím:
Immunohistochemical detection of raf protein kinase in cerebral cortical areas of adult guinea pigs and rats
Létrehozó:
Mihály, András
Endrész, Valéria
Oravecz, T.
Rapp, U. R.
Kuhnt, U.
Kiadó:
Elsevier
Dátum:
1993
Téma:
QR Microbiology / mikrobiológia
RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry / idegkórtan, neurológia, pszichiátria
Tartalmi leírás:
The raf protooncogenes are the cellular counterparts of the v-raf oncogene expressed by a murme sarcoma virus The raf protooncogenes
encode cytoplasmic serme/threonme-spectfic protein klnases which can be activated from different growth factor receptors by phosphorylatmn
The mRNAs of raf protooncogenes are found m a large variety of normal adult tissues, including the central nervous system As concerns the
dlstnbutmn and locahzatmn of their protem products (the raf klnases), very few data are to be found m the hterature This ~s the first detailed
description of their hght m~croscoplc locahzatmn in neocort~cal and allocort~cal areas of rodents Preembeddmg immunoh~stochem~cal studies
were performed on vlbratome sections from the brains of adult guinea pigs and albino rats The locahzatmns of two lsoenzymes, raf-1 kmase and
B-raf lanase, were studied with the help of lsoenzyme-specfflC polyclonal antibodies Both of the antibodies detected raf proteln-hke
tmmunoreactw~ty m many neurons and scattered ghal cells of the sensory neocortex, and the cmgular, pyriform, penrhmal and entorhlnal
allocortlcal areas Pyramidal and non-pyramidal cells of Ammon's horn, granule cells of the dentate fascia and the large neurons m the hllar
regmn were immunoreactwe, too The findings indicated that B-raf protein kmase and raf-1 klnase are present almost ubiquitously m the
neurons of the investigated cortical structures The mtenslty of staining obtained w~th serial dfluttons of the antibodies indicated that the
cytoplasmic concentratmn of B-raf kmase is tended to be higher than that of raf-1 klnase The present findings suggested that the raf kmases
are locahzed m postsynaptlc structures, mainly m dendrites and cell bodies Their cytosohc locahzatlon and their abdlty to undergo mtracellular
translocatmn during actlvatmn and phosphorylatmn raise the possibility that they play a pwotal role m the mtracellular signalling of neurons
Típus:
Article
PeerReviewed
Formátum:
text
Azonosító:
Mihály, András and Endrész, Valéria and Oravecz, T. and Rapp, U. R. and Kuhnt, U. (1993) Immunohistochemical detection of raf protein kinase in cerebral cortical areas of adult guinea pigs and rats. BRAIN RESEARCH, 627 (2). pp. 225-238. ISSN 0006-8993
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