EPIGENETIC EFFECTS OF DIETARY BUTYRATE ON HEPATIC HISTONE ACETYLATION AND ENZYMES OF BIOTRANSFORMATION IN CHICKEN
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Tartalom: http://real.mtak.hu/6224/
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published

Type = Article
Cím:
EPIGENETIC EFFECTS OF DIETARY BUTYRATE ON HEPATIC HISTONE ACETYLATION AND ENZYMES OF BIOTRANSFORMATION IN CHICKEN
Létrehozó:
MĂĄtis, GĂĄbor
NeogrĂĄdy, Zsuzsanna
CsikĂł, GyĂśrgy
GĂĄlfi, PĂŠter
FĂŠbel, Hedvig
Jemnitz, Katalin
Veres, Zsuzsanna
KulcsĂĄr, Anna
KenÊz, Ákos
Huber, Korinna
Kiadó:
AkadĂŠmiai KiadĂł
Dátum:
2013
Téma:
SF Animal culture / ĂĄllattenyĂŠsztĂŠs
SV Veterinary science / ĂĄllatorvostudomĂĄny
Tartalmi leírás:
The aim of the study was to investigate the in vivo epigenetic influences of
dietary butyrate supplementation on the acetylation state of core histones and the
activity of drug-metabolising microsomal cytochrome P450 (CYP) enzymes in
the liver of broiler chickens in the starter period. One-day-old Ross 308 broilers
were fed a starter diet without or with sodium butyrate (1.5 g/kg feed) for 21 days.
After slaughtering, nucleus and microsome fractions were isolated from the exsanguinated
liver by multi-step differential centrifugation. Histone acetylation level
was detected from hepatocyte nuclei by Western blotting, while microsomal CYP
activity was examined by specific enzyme assays. Hyperacetylation of hepatic
histone H2A at lysine 5 was observed after butyrate supplementation, providing
modifications in the epigenetic regulation of cell function. No significant changes
could be found in the acetylation state of the other core histones at the acetylation
sites examined. Furthermore, butyrate did not cause any changes in the drugmetabolising
activity of hepatic microsomal CYP2H and CYP3A37 enzymes,
which are mainly involved in the biotransformation of most xenobiotics in
chicken. These data indicate that supplementation of the diet with butyrate probably
does not have any pharmacokinetic interactions with simultaneously applied
xenobiotics.
Nyelv:
magyar
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Måtis, Gåbor and Neogrådy, Zsuzsanna and Csikó, GyÜrgy and Gålfi, PÊter and FÊbel, Hedvig and Jemnitz, Katalin and Veres, Zsuzsanna and Kulcsår, Anna and KenÊz, Ákos and Huber, Korinna (2013) EPIGENETIC EFFECTS OF DIETARY BUTYRATE ON HEPATIC HISTONE ACETYLATION AND ENZYMES OF BIOTRANSFORMATION IN CHICKEN. Acta Veterinaria Hungarica, 61 (4). pp. 477-490. ISSN 0236-6290 (print), 1588-2705 (online)
Kapcsolat:
DOI: 10.1556/AVet.2013.033