NDA@SZTAKI

The effect of nerve growth factor on nuclear progression of porcine oocytes during in vitro maturation and embryo development

Bali-Papp, Ágnes

Somfai, TamĂĄs

Tartaglione, M.

Varga, Erika

Gardon, J. C.

AkadĂŠmiai KiadĂł

2005

SF Animal culture / ĂĄllattenyĂŠsztĂŠs

The present study examined the effect of nerve growth factor (NGF) on in vitro maturation (IVM), in vitro fertilisation (IVF) and subsequent embryonic development of porcine oocytes. Cumulus-oocyte complexes were cultured with or without 1.0 ng/ml NGF for 40 h. After IVF, they were cultured in vitro for 6 days. After 10 and 20 h of IVM, there was no difference in nuclear status between the NGF-treated and control oocytes. Significant differences were detected in nuclear progression of oocytes matured in the presence or absence of NGF at 30 h of culture. A higher proportion of NGF-treated oocytes were at M-II stage compared to the control. Nevertheless, at the end of the 40-h IVM period, there was no difference in the proportion of M-II stage oocytes between the NGF-treated and control groups. NGF in IVM medium did not influence the developmental competence of putative embryos. Most embryos remained at the 2- to 4-cell stage; however, a significant amount of embryos reached the morula stage both in the NGF and the control groups. These results suggest that NGF during IVM accelerates nuclear progression of porcine oocytes by enhancing the post-diakinetic events of meiosis.

angol

Article

PeerReviewed

info:eu-repo/semantics/article

text

http://real.mtak.hu/6179/1/1175017.pdf

Bali-Papp, Ágnes and Somfai, Tamås and Tartaglione, M. and Varga, Erika and Gardon, J. C. (2005) The effect of nerve growth factor on nuclear progression of porcine oocytes during in vitro maturation and embryo development. ACTA VETERINARIA HUNGARICA, 53. pp. 91-101. ISSN 0236-6290

http://real.mtak.hu/6179/

http://www.akademiai.com/content/l5x607mn50638716/?genre=ar...

doi:10.1556/AVet.53.2005.1.9