Up-regulation of cerebral carbonic anhydrase by anoxic stress in piglets
  • Metaadatok
Tartalom: http://real.mtak.hu/5999/
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published



Type = Article
Cím:
Up-regulation of cerebral carbonic anhydrase by anoxic stress in piglets
Létrehozó:
Nógrádi, Antal
Domoki, Ferenc
Dégi, Rózsa
Borda, Stefánia
Pákáski, Magdolna
Szabó, András
Bari, Ferenc
Kiadó:
Wiley-Blackwell
Dátum:
2003
Téma:
QH3011 Biochemistry / biokémia
QH3015 Molecular biology / molekuláris biológia
RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry / idegkórtan, neurológia, pszichiátria
Tartalmi leírás:
The resuscitation of asphyxiated babies is associated with changes in cerebral protein synthesis that can influence the neurological outcome. Insufficient gas exchange results in rapid shifts in extracellular and intracellular pH. Carbonic anhydrase (CA) plays an important role in buffering acute changes in pH in the brain. We investigated whether asphyxia/re-ventilation influences the expression of cerebral CA isoforms (CA-II, CA-III and CA-IV) in anaesthetized newborn pigs. The cerebral cortex, hippocampus, cerebellum and retina were sampled, and prepared for either CA immunohistochemistry or CA immunoblotting from piglets subjected to asphyxia (10 min) followed by 2-4 h of re-ventilation, and also from normoxic controls. The CA immunoreactivity (IR) of all the isoforms studied was weak in the controls, apart from staining of a few oligodendrocytes in the subcortical white matter, some astrocytes in the superficial layer of the cerebral cortex, the cerebellar Purkinje cells and the retinal Muller cells that possessed moderate CA-II IR. However, asphyxia induced a marked increase in the CA IR of all isoforms in all the cerebral regions investigated and the retina after 4 h of survival. The pyramidal cells of the frontal cortex and hippocampus displayed the most conspicuous increase in CA IR. Immunoblotting confirmed increased levels of all the CA isoenzymes. We conclude that raised CA levels after asphyxia may contribute to the compensatory mechanisms that protect against the pathological changes in the neonatal CNS.
Típus:
Article
PeerReviewed
Formátum:
text
Azonosító:
Nógrádi, Antal and Domoki, Ferenc and Dégi, Rózsa and Borda, Stefánia and Pákáski, Magdolna and Szabó, András and Bari, Ferenc (2003) Up-regulation of cerebral carbonic anhydrase by anoxic stress in piglets. JOURNAL OF NEUROCHEMISTRY, 85 (4). pp. 843-850. ISSN 0022-3042
Kapcsolat: