N-methyl-D-aspartate-induced vasodilation is mediated by endothelium-independent nitric oxide release in piglets
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Type = Article
Cím:
N-methyl-D-aspartate-induced vasodilation is mediated by endothelium-independent nitric oxide release in piglets
Létrehozó:
Domoki, Ferenc
Perciaccante, J. V.
Shimizu, K.
Bari, Ferenc
Kiadó:
American Physiological Society
Dátum:
2002
Téma:
QP Physiology / élettan
RZ Other systems of medicine / orvostudomány egyéb területei
Tartalmi leírás:
N-methyl-D-aspartate (NMDA) elicits pial arteriolar dilation that has been associated with neuronal nitric oxide (NO) production. However, endothelial factors or glial P-450 epoxygenase products may play a role. We tested whether NMDA-induced pial vasodilation 1) primarily involves NO diffusion from the parenchyma to the surface arterioles, 2) involves intact endothelial function, and 3) involves a miconazole-sensitive component. Arteriolar diameters were determined using closed cranial window-intravital microscopy in anesthetized piglets. NMDA (10-100 muM) elicited virtually identical dose-dependent dilations in paired arterioles (r = 0.94, n = 15). However, NMDA- but not bradykinin (BK)-induced dilations of arteriolar sections over large veins were reduced by 31 +/- 1% (means +/- SE, P < 0.05, n = 4) compared with adjacent sections on the cortical surface. Also, 100 &mu;M NMDA increased cerebrospinal fluid levels of NO metabolites from 3.7 +/- 1.0 to 5.3 +/- 1.2 &mu;M (P < 0.05, n = 6). Endothelial stunning by intracarotid injection of phorbol 12,13-dibutyrate did not affect NMDA- induced vasodilation but attenuated vascular responses to hypercapnia and BK by similar to70% (n = 7). Finally, miconazole (n = 6, 20 muM) pretreatment and coapplication with NMDA did not alter vascular responses to NMDA. In conclusion, NMDA appears to dilate pial arterioles exclusively through release and diffusion of NO from neurons to the pial surface in piglets.
Típus:
Article
PeerReviewed
Formátum:
text
Azonosító:
Domoki, Ferenc and Perciaccante, J. V. and Shimizu, K. and Bari, Ferenc (2002) N-methyl-D-aspartate-induced vasodilation is mediated by endothelium-independent nitric oxide release in piglets. AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 282 (4). H1404-H1409. ISSN 0363-6135
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