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Ischemia increases prostaglandin H synthase-2 levels in retina and visual cortex in piglets |
Tartalom: | http://real.mtak.hu/5994/ |
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Archívum: | MTA Könyvtár |
Gyűjtemény: |
Status = Published
Type = Article |
Cím: |
Ischemia increases prostaglandin H synthase-2 levels in retina and visual cortex in piglets
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Létrehozó: |
Dégi, Rózsa
Thore, C.
Bari, Ferenc
Nógrádi, Antal
Domoki, Ferenc
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Kiadó: |
Springer
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Dátum: |
2001
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Téma: |
RE Ophthalmology / szemészet
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Tartalmi leírás: |
Background: Ischemia increases levels of prostaglandin H synthase-2 (PGHS-2) in neonatal brain and cerebral vasculature, but effects on the developing visual system are unknown. We examined the effects of ischemia on PGHS-2 mRNA and protein levels in the retina and visual cortex in anesthetized piglets. Methods: Ten minutes of complete retinal and brain ischemia was induced by increasing intracranial pressure. After 2-12 h of reperfusion, samples of retina and visual cortex were collected for determinations of levels of PGHS-2 mRNA (RNase protection assay) or protein (immunohistochemistry and western blotting). Tissues also were obtained from control animals. Results: Levels of PGHS-2 mRNA were undetectable in control animals but showed a dramatic increase at 2-4 h in the cortex and retina in animals exposed to ischemia. Detectable but limited PGHS-2 immunoreactivity (IR) was present in the retina and visual cortex from control animals. In piglets not subjected to ischemia, PGHS-2 IR was localized mainly to the outer limiting membrane and to the Muller cells. Ischemia induced a marked increase in PGHS-2 IR in the neural retina, with the greatest increase in the photoreceptor layer. PGHS-2 levels in whole retina also increased at 8 h after ischemia. In the intact visual cortex PGHS-2 IR was evident in layers II and V. Ischemia increased the intensity of IR in layers II/III as well as layer V. Conclusions: Detectable amounts of PGHS-2 protein are present in the piglet retina and visual cortex under normal conditions, but levels are markedly increased 8-12 h after ischemic stress. Enhanced PGHS-2 levels after ischemic stress may contribute to delayed pathological changes of the visual system in the neonate.
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Típus: |
Article
PeerReviewed
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Formátum: |
text
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Azonosító: |
Dégi, Rózsa and Thore, C. and Bari, Ferenc and Nógrádi, Antal and Domoki, Ferenc (2001) Ischemia increases prostaglandin H synthase-2 levels in retina and visual cortex in piglets. GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY, 239 (1). pp. 59-65. ISSN 0721-832X
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