| Tartalom: |
http://www.sciencedirect.com/science/journal/0165022X |
| Archívum: |
MTA Könyvtár |
| Gyűjtemény: |
Status = Published
Type = Article
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| Cím: |
Preparation of Chitosan Particles Suitable for Enzyme Immobilization
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| Létrehozó: |
Biró, E.
Szemesné Németh, Ágnes
Sisak, Cs.
Feczkó, Tivadar
Gyenis, János
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| Kiadó: |
Elsevier
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| Dátum: |
2008
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| Téma: |
QH3011 Biochemistry / biokémia
QH3015 Molecular biology / molekuláris biológia
QH3020 Biophysics / biofizika
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| Tartalmi leírás: |
Macro-, micro- and nanosized chitosan particles suitable as immobilization carriers were prepared by precipitation, emulsion cross-linking and
ionic gelation methods, respectively. Effects of particle preparation parameters on particle size were investigated. Activities of ?-galactosidase
covalently attached to differently sized particles have been evaluated and compared. The highest activity was shown by the biocatalyst
immobilized on nanoparticles obtained by means of the ionotropic gelation method with sodium sulphate as gelation agent. ?-Galactosidase fixed
on macro- and microspheres exhibited excellent storage stability in aqueous solution, with no more than 5% loss of activity after 3 weeks storage
at 4 °C and pH 7.0.
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| Típus: |
Article
PeerReviewed
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| Formátum: |
text
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| Azonosító: |
Biró, E. and Szemesné Németh, Ágnes and Sisak, Cs. and Feczkó, Tivadar and Gyenis, János (2008) Preparation of Chitosan Particles Suitable for Enzyme Immobilization. JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 70 (6). pp. 1240-1246. ISSN 0165-022X
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