Different gene expression patterns in bone tissue of aging postmenopausal osteoporotic and non-osteoporotic women.
  • Metaadatok
Tartalom: http://link.springer.com/article/10.1007%2Fs00223-007-9092-3
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published


Type = Article
Cím:
Different gene expression patterns in bone tissue of aging postmenopausal osteoporotic and non-osteoporotic women.
Létrehozó:
Balla, Bernadett
Kósa, János
Kiss, János
Borsy, Adrienn Éva
Podani, János
Lazáry, Áron
Bácsi, Krisztián
Nagy, Zsolt
Takács, István
Speer, Gábor
Orosz, László
Lakatos, Péter
Kiadó:
Springer
Dátum:
2008
Téma:
RC Internal medicine / belgyógyászat
RZ Other systems of medicine / orvostudomány egyéb területei
Tartalmi leírás:
Purpose To identify genes that are differently expressed in
osteoporotic and non-osteoporotic human bone and to describe the
relationships between these genes using multivariate data analysis.
Methods Seven bone tissue samples from postmenopausal osteoporotic
patients and 10 bone tissue samples from postmenopausal
non-osteoporotic women were examined in our study. Messenger RNA was
prepared from each sample and reverse transcribed to cDNA. The
expression differences of 87 selected genes were analyzed in a Taqman
probe-based quantitative real-time RT-PCR system.
Results A Mann-Whitney U-test indicated significant differences in the
expression of nine genes (p <= 0.05). Seven of these nine genes-ALPL,
COL1A1, MMP2, MMP13, MMP9, PDGFA, NFKB1-were significantly
downregulated in the bone tissue of osteoporotic women, while CD36 and
TWIST2 were significantly upregulated in osteoporotic patients.
Principal components analysis was used to evaluate data structure and
the relationship between osteoporotic and non-osteoporotic phenotypes
based on the multiple mRNA expression profiles of 78 genes. Canonical
variates analysis demonstrated further that osteoporotic and
non-osteoporotic tissues can be distinguished by expression analysis of
genes coding growth factors/non-collagen matrix molecules, and genes
belonging to the canonical TGFB pathway.
Conclusion Significant differences observed in gene expression profiles
of osteoporotic and non-osteoporotic human bone tissues provide further
insight into the pathogenesis of this disease. Characterization of the
differences between osteoporotic and non-osteoporotic bones by
expression profiling will contribute to the development of diagnostic
tools in the future.
Típus:
Article
PeerReviewed
Formátum:
text
Azonosító:
Balla, Bernadett and Kósa, János and Kiss, János and Borsy, Adrienn Éva and Podani, János and Lazáry, Áron and Bácsi, Krisztián and Nagy, Zsolt and Takács, István and Speer, Gábor and Orosz, László and Lakatos, Péter (2008) Different gene expression patterns in bone tissue of aging postmenopausal osteoporotic and non-osteoporotic women. CALCIFIED TISSUE INTERNATIONAL, 82 (1). pp. 12-26. ISSN 0171-967X
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