| Tartalom: |
http://real.mtak.hu/5559/ |
| Archívum: |
MTA Könyvtár |
| Gyűjtemény: |
Status = Published
Type = Article
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| Cím: |
Agrobacterium-mediated barley transformation.
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| Létrehozó: |
Éva, Csaba
Csóti, László
Tamás, László
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| Kiadó: |
Szegedi Tudományegyetem
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| Dátum: |
2008
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| Téma: |
QH3015 Molecular biology / molekuláris biológia
S1 Agriculture (General) / mezőgazdaság általában
SB Plant culture / növénytermesztés
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| Tartalmi leírás: |
More than ten years have passed since the first successful Agrobacterium-mediated
barley transformation experiment, however it is still quite challenging to establish a stably
functioning agroinfiltration protocol. Efficiency of the method depends mainly on the transformation
and co-cultivation conditions, and also the components of the tissue-culture media.
With the use of an optimized media we have been able to set up a reliable, properly functioning
transformation protocol. The first generation of transgenic barley plants, transformed with a
transformation cassette carrying an aldo-keto-reductase gene from Arabidopsis thaliana and
the hpt marker gene, were analyzed at nucleic acid (both DNA and RNA) and at protein levels.
The key factors of success proved to be the use of Silwet L-77 (surfactant) in transformation
inoculum, the Cu-content of regenerating media and the continuous visual monitoring of the
transformed callus during the somatic embryogenesis
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| Típus: |
Article
PeerReviewed
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| Formátum: |
text
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| Azonosító: |
Éva, Csaba and Csóti, László and Tamás, László (2008) Agrobacterium-mediated barley transformation. ACTA BIOLOGICA SZEGEDIENSIS, 52 (1). pp. 49-51. ISSN 1588-385X
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