AFLP analysis and improved phytoextraction capacity of transgenic gshI-poplar clones (Populus canescens L.) in vitro
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Tartalom: http://www.znaturforsch.com/ac/v60c/c60c.htm
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published




Type = Article
Cím:
AFLP analysis and improved phytoextraction capacity of transgenic gshI-poplar clones (Populus canescens L.) in vitro
Létrehozó:
Gyulai, Gábor
Humphreys, M.
Bittsánszky, András
Kiss, József
Gullner, Gábor
Szabó, Zoltán
Radimszky, László
Kőmíves, Tamás
Heszky, László
Kiadó:
Verlag der Zeitschrift fur Naturforschung
Dátum:
2005
Téma:
QH3011 Biochemistry / biokémia
QH3015 Molecular biology / molekuláris biológia
RM Therapeutics. Pharmacology / terápia, gyógyszertan
RS Pharmacy and materia medica / gyógyszerészet, gyógyászati eszközök
Tartalmi leírás:
Clone stability and in vitro phytoextraction capacity of
vegetative clones of R x canescens (2n = 4x = 38) including two
transgenic clones (ggs11 and lgl6) were studied as in vitro leaf
disc cultures. Presence of the gshI-transgene in the transformed
clones was detected in PCR reactions using gshI-specific
primers. Clone stability was determined by fAFLP (fluorescent
amplified DNA fragment length polymorphism) analysis. In total,
682 AFLP fragments were identified generated by twelve selective
primer pairs after EcoRI-MseI digestion. Four fragments
generated by EcoAGT-MseCCC were different (99.4% genetic
similarity) which proves an unexpectedly low bud mutation
frequency in R x canescens. For the study of phytoextraction
capacity leaf discs (8 mm) were exposed to a concentration
series of ZnSO4 (10(-1) to 10(-5) m) incubated for 21 days on
aseptic tissue culture media WPM containing 1 mu m Cu. Zn2+
caused phytotoxicity only at high concentrations (10(-1) to 10(-
2) m). The transgenic poplar cyt-ECS (ggs11) clone, as
stimulated by the presence of Zn, showed elevated heavy metal
(Cu) uptake as compared to the non-transformed clone. These
results suggest that gshI-transgenic poplars may be suitable for
phytoremediation of soils contaminated with zinc and copper.
Típus:
Article
PeerReviewed
Formátum:
text
Azonosító:
Gyulai, Gábor and Humphreys, M. and Bittsánszky, András and Kiss, József and Gullner, Gábor and Szabó, Zoltán and Radimszky, László and Kőmíves, Tamás and Heszky, László (2005) AFLP analysis and improved phytoextraction capacity of transgenic gshI-poplar clones (Populus canescens L.) in vitro. ZEITSCHRIFT FÜR NATURFORSCHUNG C - A JOURNAL OF BIOSCIENCES, 60. pp. 300-306. ISSN 0939-5075
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