Differential expression of D-type cyclins in HaCaT keratinocytes and in psoriasis
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Tartalom: http://real.mtak.hu/5272/
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published

Type = Article
Cím:
Differential expression of D-type cyclins in HaCaT keratinocytes and in psoriasis
Létrehozó:
Belső, Nóra
Széll, Márta
Pivarcsi, A.
Kormos, Bernadett
Kenderessy Szabó, Anna
Dobozy, Attila
Kemény, Lajos
Csörgő Sándorné Bata, Zsuzsanna
Kiadó:
Nature Publishing Group
Dátum:
2008
Téma:
RL Dermatology / bőrgyógyászat
Tartalmi leírás:
In this study, we show that the G(0)-G(1)/S phase of HaCaT keratinocyte
cell cycle is characterized by D1-type cyclin expression, whereas
during the repeated rapid turnover of highly proliferating cells, the
expression of cyclins D2 and D3 dominates. Knocking down cyclin D1 mRNA
resulted in no change of cell proliferation and morphology, indicating
that D2 and D3 cyclins could substitute for D1 in driving the cell
cycle. Increased numbers of cyclin D1-expressing keratinocytes were
found in the basal layers of the lesional psoriatic epidermis compared
to both normal and non-lesional epidermis without increased expression
of cyclin D1 mRNA, suggesting a possible dysfunction in the degradation
of cyclin D1 protein. We also detected a significant increase in cyclin
D2 and D3 mRNA expressions in psoriatic epidermis compared to normal
epidermis with no difference in protein expressions. Blocking alpha
5-integrin function by a neutralizing antibody in HaCaT keratinocytes
downregulated the expression of cyclin D1 mRNA without affecting the
expressions of cyclin D2 and D3 indicating a regulatory role for alpha
5-integrin in the expression of cyclin D1. Our data suggest a possible
role for D-type cyclins in the excessive basal-cell proliferation and
perturbed keratinocyte differentiation in the psoriatic epidermis.
Típus:
Article
PeerReviewed
Formátum:
text
Azonosító:
Belső, Nóra and Széll, Márta and Pivarcsi, A. and Kormos, Bernadett and Kenderessy Szabó, Anna and Dobozy, Attila and Kemény, Lajos and Csörgő Sándorné Bata, Zsuzsanna (2008) Differential expression of D-type cyclins in HaCaT keratinocytes and in psoriasis. JOURNAL OF INVESTIGATIVE DERMATOLOGY, 128 (3). pp. 634-642. ISSN 0022-202X
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