Nanometer-scale organization of the alpha subunits of the receptors for IL2 and IL15 in human T lymphoma cells
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Tartalom: http://real.mtak.hu/5262/
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published

Type = Article
Cím:
Nanometer-scale organization of the alpha subunits of the receptors for IL2 and IL15 in human T lymphoma cells
Létrehozó:
Bakker, B. I. de
Bodnár, Andrea
Dijk, E. M. H. P. van
Vámosi, György
Damjanovich, Sándor
Jenei, Attila
Kiadó:
Company of Biologists
Dátum:
2008
Téma:
QH3020 Biophysics / biofizika
Tartalmi leírás:
Interleukin 2 and interleukin 15 (IL2 and IL15, respectively) provide
quite distinct contributions to T-cell-mediated immunity, despite
having similar receptor composition and signaling machinery. As most of
the proposed mechanisms underlying this apparent paradox attribute key
significance to the individual alpha-chains of IL2 and IL15 receptors,
we investigated the spatial organization of the receptors IL2R alpha
and IL15R alpha at the nanometer scale expressed on a human CD4(+)
leukemia T cell line using single-molecule-sensitive near-field
scanning optical microscopy (NSOM). In agreement with previous
findings, we here confirm clustering of IL2R alpha and IL15R alpha at
the submicron scale. In addition to clustering, our single-molecule
data reveal that a non-negligible percentage of the receptors are
organized as monomers. Only a minor fraction of IL2R alpha molecules
reside outside the clustered domains, whereas similar to 30% of IL15R
alpha molecules organize as monomers or small clusters, excluded from
the main domain regions. Interestingly, we also found that the packing
densities per unit area of both IL2R alpha and IL15R alpha domains
remained constant, suggesting a 'building block' type of assembly
involving repeated structures and composition. Finally, dual-color NSOM
demonstrated co-clustering of the two alpha-chains. Our results should
aid understanding the action of the IL2R-IL15R system in T cell
function and also might contribute to the more rationale design of
IL2R- or IL15R-targeted immunotherapy agents for treating human
leukemia.
Típus:
Article
PeerReviewed
Formátum:
text
Azonosító:
Bakker, B. I. de and Bodnár, Andrea and Dijk, E. M. H. P. van and Vámosi, György and Damjanovich, Sándor and Jenei, Attila (2008) Nanometer-scale organization of the alpha subunits of the receptors for IL2 and IL15 in human T lymphoma cells. JOURNAL OF CELL SCIENCE, 121 (5). pp. 627-633. ISSN 0021-9533
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