Viability and fertilizing capacity of cryopreserved sperm from three North American acipenseriform species: a retrospective study
  • Metaadatok
Tartalom: http://dx.doi.org/10.1111/j.1439-0426.2008.01134.x
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published

Type = Article
Cím:
Viability and fertilizing capacity of cryopreserved sperm from three North American acipenseriform species: a retrospective study
Létrehozó:
Horváth, Ákos
Wayman, W. R.
Dean, J. C.
Urbányi, Béla
Tiersch, T. R.
Mims, S. D.
Johnson, D.
Jenkins, J. A.
Kiadó:
Blackwell Publishing
Dátum:
2008
Téma:
SH Aquaculture. Fisheries. Angling / vizi növény-és állattartás, halászat, sporthorgászat
SV Veterinary science / állatorvostudomány
Tartalmi leírás:
Populations of sturgeon across the globe are threatened due to
unregulated harvest and habitat loss, and the status varies
among species across North America. Ready access to viable
and functional sperm would contribute to recovery programmes
for these species. In this study, we examined the
motility, viability (cell membrane integrity) of cryopreserved
sperm from three North American acipenseriform species and
fertilizing capacity. Milt samples were collected from captive
shortnose sturgeon (Acipenser brevirostrum), wild paddlefish
(Polyodon spathula) and pallid sturgeon (Scaphirhynchus albus)
and cryopreserved using combinations of Modified Tsvetkova
?s (MT) extender, Original Tsvetkova?s extender, and
modified Hanks? balanced salt solution, along with the
cryoprotectants methanol (MeOH) or dimethyl sulfoxide
(DMSO). A dual-staining technique using the fluorescent
stains SYBR-14 and propidium iodide was employed with flow
cytometry to determine the percentages of spermatozoa that
were viable by virtue of having intact membranes. The
percentage of viable spermatozoa ranged from 5% to 12%
in shortnose sturgeon, 30?59% in paddlefish, and 44?58% in
pallid sturgeon. In the first experiment with shortnose sturgeon
sperm, methanol allowed for higher values for dependent
variables than did DMSO, and sperm viability generally
correlated with post-thaw motility. However, fertilization rate,
neurulation, or hatching rates were independent from these
factors. In the second experiment with shortnose sturgeon, 5%
MeOH combined with MT yielded higher values for all
parameters tested than the other combinations: viability was
correlated with motility, fertilization rate, and hatching rate.
Overall, viability and post-thaw motility was not affected by
the use of hyperosmotic extenders (OT) or cryoprotectants
(DMSO), but their use decreased fertilization percentages. For
paddlefish sperm (experiment 3), MT combined with 10%
MeOH was clearly a good choice for cryopreservation;
viability and motility results were correlated, but independent
of fertilization. For pallid sturgeon sperm (experiment 4), MT
with 5?10% MeOH showed significantly higher sperm quality
and fertilization parameters. Membrane integrity can be used
as a predictor of fertilization by cryopreserved sperm, however
additional sperm quality parameters, supplementary to motility
and membrane integrity, would be useful in the refining and
optimizing cryopreservation protocols with acipenseriform
sperm.
Típus:
Article
PeerReviewed
Formátum:
text
Azonosító:
Horváth, Ákos and Wayman, W. R. and Dean, J. C. and Urbányi, Béla and Tiersch, T. R. and Mims, S. D. and Johnson, D. and Jenkins, J. A. (2008) Viability and fertilizing capacity of cryopreserved sperm from three North American acipenseriform species: a retrospective study. Journal of Applied Ichthyology, 24 (4). pp. 443-449. ISSN 0175-8659 (print), 1439-0426 (online)
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