The relationship of the cryoprotectants methanol and dimethyl sulfoxide and hyperosmotic extenders on sperm cryopreservation of two North-American sturgeon species
  • Metaadatok
Tartalom: http://dx.doi.org/10.1016/j.aquaculture.2005.02.007
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published


Type = Article
Cím:
The relationship of the cryoprotectants methanol and dimethyl sulfoxide and hyperosmotic extenders on sperm cryopreservation of two North-American sturgeon species
Létrehozó:
Horváth, Ákos
Wayman, William R.
Urbányi, Béla
Ware, Kent M.
Dean, Jan C.
Tiersch, Terrence R.
Kiadó:
Elsevier
Dátum:
2005
Téma:
SF Animal culture / állattenyésztés
SH Aquaculture. Fisheries. Angling / vizi növény-és állattartás, halászat, sporthorgászat
Tartalmi leírás:
Successful sperm cryopreservation techniques have been developed for Eurasian sturgeon species; however, there is little
information available on these techniques for North-American species. In this study, two sets of sperm cryopreservation
experiments were carried out on the endangered shortnose sturgeon (Acipenser brevirostrum). In the first set, the
cryoprotectants methanol (MeOH) and dimethyl sulfoxide (DMSO) were investigated using three concentrations (5%, 10%
and 15%). The highest post-thaw motility was found using 5% DMSO (26F13%) while the use of 5% MeOH resulted in the
highest rates for fertilization at the 4-cell stage (40F15%), neurulation (38F13%) and hatching (32F12%). In the second set,
the Original Tsvetkova?s extender (OT), Modified Tsvetkova?s extender (MT) and modified Hanks? balanced salt solution
(mHBSS) were investigated in combination with three MeOH concentrations. The highest post-thaw motility (18F10%),
fertilization (18F11%) and hatching rates (17F12%) were observed with MT extender used in combination with 5% MeOH.
In another set of experiments, the effects of two extenders (MT and mHBSS) and two concentrations of MeOH were
investigated for sperm cryopreservation of pallid sturgeon (Scaphyrinchus albus). The highest post-thaw motility (70F10%)
was observed using MT and 10% MeOH while MT and 5% MeOH yielded the highest rates of fertilization (88F6%) and hatching (73F14%). In general we conclude that although hyperosmotic conditions of extenders and cryoprotectants result in
higher post-thaw motility, they seem to reduce the fertilizing ability of the sperm.
Típus:
Article
PeerReviewed
Formátum:
text
Azonosító:
Horváth, Ákos and Wayman, William R. and Urbányi, Béla and Ware, Kent M. and Dean, Jan C. and Tiersch, Terrence R. (2005) The relationship of the cryoprotectants methanol and dimethyl sulfoxide and hyperosmotic extenders on sperm cryopreservation of two North-American sturgeon species. Aquaculture, 247 (1-4). pp. 243-251. ISSN 0044-8486
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