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Bejelentkezés
Kapcsolat
Cryopreservation of common carp sperm |
Tartalom: | http://dx.doi.org/10.1016/S0990-7440(03)00084-6 |
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Archívum: | MTA Könyvtár |
Gyűjtemény: |
Status = Published
Type = Article |
Cím: |
Cryopreservation of common carp sperm
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Létrehozó: |
Horváth, Ákos
Miskolczi, Edit
Urbányi, Béla
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Kiadó: |
EDP Sciences
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Dátum: |
2003
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Téma: |
SF Animal culture / állattenyésztés
SH Aquaculture. Fisheries. Angling / vizi növény-és állattartás, halászat, sporthorgászat
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Tartalmi leírás: |
Experiments were carried out to investigate the effect of five extenders (sucrose, glucose, fructose, KCl and a saline carp sperm extender)
and two cryoprotectants (dimethyl-sulfoxide (DMSO) and methanol) on the cryopreservation of common carp sperm. Freezing of sperm using glucose extender and methanol as cryoprotectant resulted in the highest post-thaw motility, fertilization as well as hatching rates (63 ± 9%, 74 ± 15% and 67 ± 17% vs. 87 ± 5%, 84 ± 14% and 69 ± 14% using fresh sperm, respectively). In general, sugar-based extenders combined with methanol as cryoprotectant yielded higher motility, fertilization and hatching rates than ionic extenders in combination with DMSO. The jelly-like agglutination observed after thawing in samples frozen with sugar-based extenders did not reduce fertilization and hatching rates. Frozen?thawed sperm samples were able to successfully fertilize 10 g (8000) eggs. |
Típus: |
Article
PeerReviewed
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Formátum: |
text
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Azonosító: |
Horváth, Ákos and Miskolczi, Edit and Urbányi, Béla (2003) Cryopreservation of common carp sperm. Aquatic Living Resources, 16 (5). pp. 457-460. ISSN 0990-7440
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Kapcsolat: |
http://dx.doi.org/10.1016/S0990-7440(03)00084-6
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