Fertilization rate of Siberian sturgeon (Acipenser baeri, Brandt) milt cryopreserved with methanol
  • Metaadatok
Tartalom: http://dx.doi.org/10.1016/S0044-8486(02)00003-0
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published


Type = Article
Cím:
Fertilization rate of Siberian sturgeon (Acipenser baeri, Brandt) milt cryopreserved with methanol
Létrehozó:
Glogowski, J.
Kolman, R.
Szczepkowski, M.
Horváth, Ákos
Urbányi, Béla
Sieczyński, P.
Rzemieniecki, A.
Domagała, J.
Demianowicz, W.
Kowalski, R.
Ciereszko, A.
Kiadó:
Elsevier
Dátum:
2002
Téma:
SF Animal culture / állattenyésztés
SH Aquaculture. Fisheries. Angling / vizi növény-és állattartás, halászat, sporthorgászat
Tartalmi leírás:
Milt obtained from three Siberian sturgeon males (Acipenser baeri, Brandt) were cryopreserved using three extenders: Tris?sucrose?KCl (30 mM Tris, 23.4 mM sucrose, 0.25 mM KCl, pH 8.0), Tris?NaCl (10 mM Tris, 25 mM NaCl, pH 8.5), and Tris?sucrose (20 mM Tris, 400 mM sucrose, pH 8.0) supplemented with 10% methanol. Semen was diluted 1:1 with appropriate extender and frozen in liquid nitrogen vapor. After fertilization with cryopreserved milt, hatching rates of 29.6±5.0%, 18.2±2.4%, and 6.0±3.0% were recorded for Tris?sucrose?KCl, Tris?NaCl, and Tris?sucrose extender, respectively. Rates for the first two extenders were similar to data of fresh semen obtained from two males(17.9% and 26.0% for male #1 and #2, respectively). Our results indicate that Tris?sucrose?KCl and Tris?NaCl are useful extenders and methanol is a useful cryoprotectant for cryopreservation of sturgeon semen.
Típus:
Article
PeerReviewed
Formátum:
text
Azonosító:
Glogowski, J. and Kolman, R. and Szczepkowski, M. and Horváth, Ákos and Urbányi, Béla and Sieczyński, P. and Rzemieniecki, A. and Domagała, J. and Demianowicz, W. and Kowalski, R. and Ciereszko, A. (2002) Fertilization rate of Siberian sturgeon (Acipenser baeri, Brandt) milt cryopreserved with methanol. Aquaculture, 211 (1-4). pp. 367-373. ISSN 0044-8486
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