Within-host evolution of Brucella canis during a canine brucellosis outbreak in a kennel
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Tartalom: http://www.biomedcentral.com/1746-6148/9/76
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published
Type = Article
Cím:
Within-host evolution of Brucella canis during a canine brucellosis outbreak in a kennel
Létrehozó:
Gyuranecz, Miklós
Rannals, Brandy D.
Allen, Christina A.
Jánosi, Szilárd
Keim, Paul S.
Foster, Jeffrey T.
Dátum:
2013
Téma:
SV Veterinary science / állatorvostudomány
Tartalmi leírás:
Abstract
Background: Little is currently known about Brucella evolution within the host during infection. The current study
is the first to employ fine-scale genotyping on an isolate collection derived from a Brucella canis outbreak. Eight
isolates of B. canis, cultured from different tissues of three dogs (female, stud dog, puppy of another female) from a
single kennel over three months were genetically characterized with a 15-marker multi-locus, variable-number
tandem repeat (VNTR) analysis (MLVA) to assess the genetic relatedness of isolates and potential rapid mutational
changes.
Results: MLVA discriminated among the otherwise indistinguishable isolates from different animals and from
isolates collected at different time points within each host, with different VNTR alleles being detected at multiple
dates and tissue sites. We suspect that all isolates cultured from the female, puppy, and stud dogs originated from
the same strain, with subsequent rapid in vivo mutations. However, high mutation rates and apparent in several of
the loci prevented making definitive epidemiological relationships among isolates.
Conclusions: This investigation highlights the rapid in vivo genetic mutations of several VNTRs of B. canis over a
short time period in the host and the emergence of alternate alleles. However, this work also suggests the
challenges of using highly mutable VNTRs to infer epidemiological relationships of strains within a short duration
outbreak.
Keywords: Brucella canis, Brucellosis, Evolution, MLVA, VNTR
Típus:
Article
PeerReviewed
Formátum:
text
Azonosító:
Gyuranecz, Miklós and Rannals, Brandy D. and Allen, Christina A. and Jánosi, Szilárd and Keim, Paul S. and Foster, Jeffrey T. (2013) Within-host evolution of Brucella canis during a canine brucellosis outbreak in a kennel. BMC Veterinary Research, 9. p. 76.
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