In VitroCytotoxicity, Chemotactic Effect, and Cellular Uptake of Branched Polypeptides with Poly[l-Lys] Backbone by J774 Murine Macrophage Cell Line
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Tartalom: http://real.mtak.hu/4711/
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published



Type = Article
Cím:
In VitroCytotoxicity, Chemotactic Effect, and Cellular Uptake of Branched Polypeptides with Poly[l-Lys] Backbone by J774 Murine Macrophage Cell Line
Létrehozó:
SzabĂł, Rita
Mező, Gábor
Pállinger, Éva
Kovács, Péter
Kőhidai, László
BĹ‘sze, Szilvia
Hudecz, Ferenc
Kiadó:
American Chemical Society
Dátum:
2008
Téma:
QD Chemistry / kémia
QH3011 Biochemistry / biokémia
QH3015 Molecular biology / molekuláris biológia
Tartalmi leírás:
Branched polypeptides with polylysine backbone are promising candidates for selective delivery of drugs, epitopes. or reporter molecules. We reported earlier that polylysine-based polypeptides with polyanionic character were internalized by murine bone marrow derived macrophages via class A scavenger receptor. In the present studies, our investigations were extended to seven polypeptides with different amino acid composition and charge properties. We report on our findings on the concentration-dependent influence of these compounds on survival and chemotaxis of the murine macrophage-like cell line J774 and internalization properties of the polypeptides by J774 cells. Our observations indicate that the polypeptides regardless of their charge properties were essentially nontoxic and did not alter significantly the chemotaxis of J774 cells; therefore, the polypeptides suit the requirements for nontoxic and "neutral" carrier molecules. We also demonstrated that the polypeptides were internalized efficiently by J774 cells, depending on their chemical structure and charge properties. Using the scavenger receptor-ligand fucoidan as inhibitor, we established that the scavenger receptor played a role-in accordance with findings on murine bone marrow derived macrophages in the internalization only of the polyanionic polypeptides.
Nyelv:
magyar
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Szabó, Rita and Mező, Gábor and Pállinger, Éva and Kovács, Péter and Kőhidai, László and Bősze, Szilvia and Hudecz, Ferenc (2008) In VitroCytotoxicity, Chemotactic Effect, and Cellular Uptake of Branched Polypeptides with Poly[l-Lys] Backbone by J774 Murine Macrophage Cell Line. Bioconjugate Chemistry, 19 (5). pp. 1078-1086. ISSN 1043-1802 (print), 1520-4812 (online)
Kapcsolat:
doi:10.1021/bc7004544