Supravital microwave experiments support that the formation of ?dark? neurons is propelled by phase transition in an intracellular gel system
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Tartalom: http://www.sciencedirect.com/science/article/pii/S0006899...
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published

Type = Article
Cím:
Supravital microwave experiments support that the formation of ?dark? neurons is propelled by phase transition in an intracellular gel system
Létrehozó:
Gallyas, Ferenc
Pál, József
Bukovics, Péter
Kiadó:
Elsevier
Dátum:
2009
Téma:
RB Pathology / patológia, kórtan
Tartalmi leírás:
Background: Based on circumstantial evidences, we (Gallyas, F., Farkas, O., Mázló, M., 2004.
Gel-to-gel phase transition may occur in mammalian cells: Mechanism of formation of
?dark? (compacted) neurons. Biol. Cell 96, 313?324.) proposed that the formation of ?dark?
neurons (striking compaction of visibly normal ultrastructural elements accompanied with
large-scale fluid excretion), which occur in many neurological diseases such as ischemia,
proceeds with a non-enzymatic mechanism. Objective: To support this proposition, the
present paper deals with the results of supravital experiments using microwave irradiation.
Method: After transcardial glutaraldehyde fixation followed by decapitation, a pin was stuck
into rat brains just before and just after they were warmed up to 80 °C and maintained at this
temperature for various periods of time by controlled microwave irradiation. Results:
Independently of the duration of irradiation, the pre-irradiation pin sticking produced
numerous ?dark? neurons in an approximating 500-?m-wide zone around its track whereas
the post-irradiation pin sticking did the same only when the irradiation was shorter than
55 s. The excreted fluid was present in neighbouring astrocytic processes but not in the
extracellular space. Conclusions: The formation of ?dark? neurons is completed in less than
55 s under the circumstances of the experiment. As neurons are poor in readily consumable
chemical energy in the absence of blood circulation, this rapid and massive fluid excretion
cannot be explained by any enzyme-mediated membrane-related pump mechanism. An
osmotic mechanism can also be discounted. In contrast, it is in conformity with the above
mentioned non-enzymatic (physicochemical) phenomenon, the phase transition of a supracytoskeletal
gel network storing free energy in the form of non-covalent interactions.
Típus:
Article
PeerReviewed
Formátum:
application/pdf
Azonosító:
Gallyas, Ferenc and Pál, József and Bukovics, Péter (2009) Supravital microwave experiments support that the formation of ?dark? neurons is propelled by phase transition in an intracellular gel system. Brain Research, 1270 . pp. 152-156. ISSN 0006-8993
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